昆虫学报 ›› 2016, Vol. 59 ›› Issue (8): 896-905.doi: 10.16380/j.kcxb.2016.08.012

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昆虫学的基本科学问题及分子生物学技术  

赵小凡   

  1. (山东大学生命科学学院, 济南 250100)
  • 出版日期:2016-08-20 发布日期:2016-08-20

Basic scientific questions and molecular biology techniques in entomology

ZHAO Xiao-Fan   

  1.  (School of Life Sciences, Shandong University, Jinan 250100, China)
  • Online:2016-08-20 Published:2016-08-20

摘要: 昆虫与人类生产及生活关系密切,昆虫学研究具有重要的理论意义和实践价值。昆虫容易大量饲养,是研究生物学基本科学问题的良好材料。分子生物学技术与方法的快速发展,极大地推动了昆虫学研究进程。转录组测序的普及以及基因编辑(gene editing)技术的成功已经打破了模式生物与非模式生物的界限,为研究各种重要的农业害虫提供了技术。我们应当根据生产实践的需要和现有理论凝练重要的创新性科学问题,灵活运用各种研究方法和技术,推动昆虫学研究进展,阐明昆虫学领域的特殊科学问题及大生物学领域的共同科学问题。  

关键词: 昆虫, 科学问题, 方法与技术, 转录组, 基因编辑

Abstract: Insects are closely associated with human production and life, and therefore entomological research has important theoretical significance and practical value. Insects are good materials to study biological questions because they are easily bred in a large number. The rapid development of molecular biology techniques has greatly promoted the process of entomological research. The success of the transcriptome sequencing and gene editing technologies has broken the boundary of the model species and the non-model species, providing techniques for the study of various important agricultural pests. We need to consider the important scientific questions according to the needs of production practice and the existing theories, promote entomological research progress by using various research methods and techniques, and clarify special scientific questions in the field of entomology and common scientific questions in the field of biology as well.  

Key words:  Insect, scientific questions, methods and techniques, transcriptome, gene editing